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Technology High performance liquid chromatography (HPLC)
 
Protection strategy Product authentication
Type Analytical technique
System Laboratory based
Medium Medicine
Authentication/Identificaton Authentication
Traceability No
Cost Price on request
Required equipment HPLC apparatus,
solvents
Vertification level Laboratories
N.B.
Description HPLC is a technique used to separate the different compounds of a sample.
The sample (dissolution in solvents needed) is injected in the apparatus, pushed through a thin column by the solvent, the column contains a stationary phase and the solvent is the mobile phase. The different constituents interact with the two phases, this interaction is going to determine their rate of progress. The retention time of the constituent is distinctive for a given molecule in the same analytical system. HPLC can identify and measure active ingredients and many impurities, but may not detect excipients which are not soluble in the mobile phase. Different detectors can be use: UV-visible spectroscopy, mass spectrometry, etc.. The most effective and sensitive being the combination HPLC-MS
 
Strengths Identification of the different constituents of a sample;
Can be used for profiling;
Unequivocal identification of complex pharmaceutical samples when coupled with a mass spectrometer;
 
Limitations Cost;
Requires equipment + formation;
Destructive of the sample;
Less accurate than GC-MS;
 
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